Lentiviral vectors (LVV) are commonly used as gene delivery tools for cell and gene therapies, notably chimeric antigen receptor (CAR) T cell therapies. Like other retroviruses, lentiviruses can convert their single-stranded RNA genome into double-stranded DNA when integrating into the genome of a cell. Unlike other retroviruses, lentiviruses can transduce non-dividing and quiescent cells, which makes them highly suitable for use in cell therapy. Corinne Branciaroli and Dr. Katie Roberts at OXGENE analyse the development of stable lentiviral cell lines.